三柴 研究業績

【原著論文】

  1. Kei-ichiro Mishiba, Yuji Iwata, Tomofumi Mochizuki, Atsushi Matsumura, Nanami Nishioka, Rikako Hirata, Nozomu Koizumi (2019)
    Unfolded protein-independent IRE1 activation contributes to multifaceted developmental processes in Arabidopsis.
    Life Science Alliance 2(5): e201900459
  2. Rikako Hirata, Kei-ichiro Mishiba, Nozomu Koizumi, Yuji Iwata (2019)
    Deficiency in the double-stranded RNA binding protein HYPONASTIC LEAVES1 increases sensitivity to the endoplasmic reticulum stress inducer tunicamycin in Arabidopsis.
    BMC Research Notes 12(1): 580
  3. Ayumi Hirohata, Izumi Sato, Kimihiko Kaino, Yuji Iwata, Nozomu Koizumi, Kei-ichiro Mishiba (2019)
    CRISPR/Cas9-mediated homologous recombination in tobacco.
    Plant Cell Reports 38(4): 463-473
  4. Yuji Iwata, Tsukasa Iida, Toshihiro Matsunami, Yu Yamada, Kei‐ichiro Mishiba, Takumi Ogawa, Tetsuya Kurata, Nozomu Koizumi (2018)
    Constitutive BiP protein accumulation in Arabidopsis mutants defective in a gene encoding chloroplast‐resident stearoyl‐acyl carrier protein desaturase.
    Genes to Cells 23(6): 456-465
  5. Misa Matsuda, Yuji Iwata, Nozomu Koizumi, Kei-ichiro Mishiba (2018)
    DNA double-strand breaks promote endoreduplication in radish cotyledon.
    Plant Cell Reports 37(6): 913-921
  6. Yuji Iwata, Fumika Yagi, Sae Saito, Kei-ichiro Mishiba, Nozomu Koizumi (2017)
    Inositol-requiring enzyme 1 affects meristematic division in roots under moderate salt stress in Arabidopsis.
    Plant Biotechnology 34(3): 159-163
  7. Asahi Shimada, Azusa Okumura, Satoshi Yamasaki, Yuji Iwata, Nozomu Koizumi, Masahiro Nishihara, Kei-ichiro Mishiba (2017)
    A 64-bp sequence containing the GAAGA motif is essential for CaMV-35S promoter methylation in gentian.
    Biochimica et Biophysica Acta (BBA) – Gene Regulatory Mechanisms 1860(8): 861-869
  8. Yuji Iwata, Makoto Ashida, Chisa Hasegawa, Kazuki Tabara, Kei-ichiro Mishiba, Nozomu Koizumi (2017)
    Activation of the Arabidopsis membrane-bound transcription factor bZIP28 is mediated by site-2 protease, but not site-1 protease.
    Plant Journal 91(3): 408-415
  9. Riko Tanaka, Makoto Amijima, Yuji Iwata, Nozomu Koizumi, Kei-ichiro Mishiba (2016)
    Effect of light and auxin transport inhibitors on endoreduplication in hypocotyl and cotyledon.
    Plant Cell Reports 35(12): 2539-2547
  10. Yuji Iwata, Noriko Hayashi, Kazuki Tabara, Kei-ichiro Mishiba, Nozomu Koizumi (2016)
    Tunicamycin-induced inhibition of protein secretion into culture medium of Arabidopsis T87 suspension cells through mRNA degradation on the endoplasmic reticulum.
    Bioscience, Biotechnology, and Biochemistry 80(6): 1168-1171
  11. Yukihiro Nagashima, Yuji Iwata, Kei-ichiro Mishiba, Nozomu Koizumi (2016)
    Arabidopsis tRNA ligase completes the cytoplasmic splicing of bZIP60 mRNA in the unfolded protein response.
    Biochemical and Biophysical Research Communications 470(4): 941-946
  12. Azusa Okumura, Asahi Shimada, Satoshi Yamasaki, Takuya Horino, Yuji Iwata, Nozomu Koizumi, Masahiro Nishihara, Kei-ichiro Mishiba (2016)
    CaMV-35S promoter sequence-specific DNA methylation in lettuce.
    Plant Cell Reports 35(1): 43-51
  13. Atsushi Matsumura, Kenji Yamawaki, Tatsuro Taniguchi, Arata Tarui, Mohammad Amzad Hossain, Kei-ichiro Mishiba, Hiroyuki Daimon (2014)
    Mycorrhizal status of the genus Curcuma plants cultivated on the main island of Okinawa.
    Tropical Agriculture and Development 58(4): 123-134
  14. Yukihiro Nagashima, Yuji Iwata, Makoto Ashida, Kei-ichiro Mishiba, Nozomu Koizumi (2014)
    Exogenous salicylic acid activates two signaling arms of the unfolded protein response in Arabidopsis.
    Plant & Cell Physiology 55(10): 1772-1778
  15. Makoto Amijima, Yuji Iwata, Nozomu Koizumi, Kei-ichiro Mishiba (2014)
    The polar auxin transport inhibitor TIBA inhibits endoreduplication in dark grown spinach hypocotyls.
    Plant Science 225: 45-51
  16. Yosuke Miyagawa, Jun Ogawa, Yuji Iwata, Nozomu Koizumi, Kei-ichiro Mishiba (2013)
    An attempt to detect siRNA-mediated genomic DNA modification by artificially induced mismatch siRNA in Arabidopsis.
    PLOS ONE 8(11): e81326
  17. Kei-ichiro Mishiba, Yukihiro Nagashima, Eiji Suzuki, Noriko Hayashi, Yoshiyuki Ogata, Yukihisa Shimada, Nozomu Koizumi (2013)
    Defects in IRE1 enhance cell death and fail to degrade mRNAs encoding secretory pathway proteins in the Arabidopsis unfolded protein response.
    Proceedings of the National Academy of Sciences of the United States of America 110(14): 5713-5718
  18. Yukihiro Nagashima, Kei-ichiro Mishiba, Eiji Suzuki, Yukihisa Shimada, Yuji Iwata, Nozomu Koizumi (2011)
    Arabidopsis IRE1 catalyses unconventional splicing of bZIP60 mRNA to produce the active transcription factor.
    Scientific Reports 1: 29
  19. Satoshi Yamasaki, Masayuki Oda, Nozomu Koizumi, Kazuhiko Mitsukuri, Masafumi Johkan, Takashi Nakatsuka, Masahiro Nishihara, Kei-ichiro Mishiba (2011)
    De novo DNA methylation of the 35S enhancer revealed by high-resolution methylation analysis of an entire T-DNA segment in transgenic gentian.
    Plant Biotechnology 28(2): 223-230
  20. Satoshi Yamasaki, Masayuki Oda, Hiroyuki Daimon, Kazuhiko Mitsukuri, Masahumi Johkan, Takashi Nakatsuka, Masahiro Nishihara, Kei-ichiro Mishiba (2011)
    Epigenetic modifications of the 35S promoter in cultured gentian cells.
    Plant Science 180(4): 612-619
  21. Mwathi Jane Wamaitha, Kyoko Suwa, Ken-ichi Fukuda, Masahiro Mii, Hiroyuki Daimon, Kei-ichiro Mishiba (2010)
    Thidiazuron-induced rapid shoot regeneration via embryo-like structure formation from shoot tip-derived callus culture of sugarcane.
    Plant Biotechnology 27(4): 365-368
  22. Kazuhiko Mitsukuri, Masahumi Johkan, Satoshi Yamasaki, Hideyuki Tanaka, Takahiro Tezuka, Kei-ichiro Mishiba, Masayuki Oda (2010)
    L-2-aminooxy-3-phenylpropionic acid (AOPP) controls browning and promotes adventitious bud formation in Neofinetia falcata in vitro.
    Journal of the Japanese Society for Horticultural Science 79(4): 367-371
  23. Kei-ichiro Mishiba*, Satoshi Yamasaki*, Takashi Nakatsuka, Yoshiko Abe, Hiroyuki Daimon, Masayuki Oda, Masahiro Nishihara (2010)
    Strict de novo methylation of the 35S enhancer sequence in gentian.
    PLOS ONE 5(3): e9670 (*equally contribution)
  24. Takashi Nakatsuka, Kei-ichiro Mishiba, Akiko Kubota, Yoshiko Abe, Saburo Yamamura, Noriko Nakamura, Yoshikazu Tanaka, Masahiro Nishihara (2010)
    Genetic engineering of novel flower colour by suppression of anthocyanin modification genes in gentian.
    Journal of Plant Physiology 167(3): 231-237
  25. Masahumi Johkan, Tomoko Chiba, Kazuhiko Mitsukuri, Satoshi Yamasaki, Hideyuki Tanaka, Kei-ichiro Mishiba, Toshinobu Morikawa, Masayuki Oda (2010)
    Seed production enhanced by antiauxin in the pat-2 parthenocarpic tomato mutant.
    Journal of the American Society for Horticultural Science 135(1): 3-8
  26. Hiroshi Ishizaka, Takayoshi Oku, Kei-ichiro Mishiba, Masahiro Mii (2009)
    Identification of ploidy level of Cyclamen rohlfsianum plants through flow cytometric and cytological analysis of C. rohlfsianum, C. purpurascens and their hybrid.
    Cytologia 74(4): 457-465
  27. Kazuhiko Mitsukuri, Masahumi Johkan, Satoshi Yamasaki, Takahiro Tezuka, Kei-ichiro Mishiba, Masayuki Oda (2009)
    Effects of anti-browning conditioning on adventitious bud formation in Neofinetia falcata Hu. in vitro.
    Horticulture Environment and Biotechnology 50(4): 295-299
  28. Kei-ichiro Mishiba, Kyoko Yamane, Takashi Nakatsuka, Yuki Nakano, Saburo Yamamura, Jun Abe, Hiromi Kawamura, Yoshihito Takahata, Masahiro Nishihara (2009)
    Genetic relationships in the genus Gentiana based on chloroplast DNA sequence data and nuclear DNA content.
    Breeding Science 59(2): 119-127
  29. Kazuhiko Mitsukuri, Genjiro Mori, Masafumi Johkan, Yukiko Shimada, Kei-ichiro Mishiba, Toshinobu Morikawa, Masayuki Oda (2009)
    Effects of explant position and dark treatment on bud formation in floret culture of Ponerorchis graminifolia Rchb.f.
    Scientia Horticulturae 121(2): 243-247
  30. Kazuhiko Mitsukuri, Genjiro Mori, Masahumi Johkan, Kei-ichiro Mishiba, Toshinobu Morikawa, Masayuki Oda (2009)
    Effects of explant source and dark-preconditioning on adventitious bud formation in Neofinetia falcata H. H. Hu in vitro.
    Journal of the Japanese Society for Horticultural Science 78(2): 252-256
  31. Kazuhiko Mitsukuri, Takaya Arita, Masahumi Johkan, Satoshi Yamasaki, Kei-ichiro Mishiba, Masayuki Oda (2009)
    Effects of type of explant and dark preconditioning on bud formation in Habenaria radiata (Thunb.) in vitro.
    HortScience 44(2): 523-525
  32. Masafumi Johkan, Genjiro Mori, Yoshihiro Imahori, Kazuhiko Mitsukuri, Satoshi Yamasaki, Kei-ichiro Mishiba, Toshinobu Morikawa, Masayuki Oda (2008)
    Shading the cut stems of tomato plants promotes in vivo shoot regeneration via control of the phenolic metabolism.
    Environment Control in Biology 46(3): 203-209
  33. Masafumi Johkan, Genjiro Mori, Kazuhiko Mitsukuri, Kei-ichiro Mishiba, Toshinobu Morikawa, Yoshihiro Imahori, Masayuki Oda (2008)
    Effect of ascorbic acid on in vivo organogenesis in tomato plants.
    Journal of Horticultural Science & Biotechnology 83(5): 624-628
  34. Yusuke Kazama, Hiroyuki Saito, Mashu Miyagi, Hinako Takehisa, Hiroyuki Ichida, Yutaka Miyazawa, Kei-ichiro Mishiba, Takeshi Kanaya, Kenichi Suzuki, Chang-Hyu Bae, Kazumitsu Miyoshi, Masahiro Mii, Tomoko Abe (2008)
    Effect of heavy ion-beam irradiation on plant growth and mutation induction in Nicotiana tabacum.
    Plant Biotechnology 25(1): 105-111
  35. Takashi Nakatsuka, Kei-ichiro Mishiba, Yoshiko Abe, Akiko Kubota, Yuko Kakizaki, Saburo Yamamura, Masahiro Nishihara (2008)
    Flower color modification of gentian plants by RNAi-mediated gene silencing.
    Plant Biotechnology 25(1): 61-68
  36. Masafumi Johkan, Genjiro Mori, Kazuhiko Mitsukuri, Kei-ichiro Mishiba, Toshinobu Morikawa, Masayuki Oda (2008)
    In vivo shoot regeneration promoted by shading the cut surface of the stem in tomato plants.
    HortScience 43(1): 220-222
  37. Dong Poh Chin, Kei-ichiro Mishiba, Masahiro Mii (2007)
    Agrobacterium-mediated transformation of protocorm-like bodies in Cymbidium.
    Plant Cell Reports 26(6): 735-743
  38. Ken-ichi Fukuda, Hiroyuki Daimon, Kei-ichiro Mishiba, Masahiro Mii (2007)
    Histological observation of root buds formation of hairy roots in Lotus corniculatus L.
    Grassland Science 53(1): 51-53
  39. Yasukazu Daigaku, Satsuki Mashiko, Kei-ichiro Mishiba, Saburo Yamamura, Ayako Ui, Takemi Enomoto, Kazuo Yamamoto (2006)
    Loss of heterozygosity in yeast can occur by ultraviolet irradiation during the S phase of the cell cycle.
    Mutation Research 600(1): 173-187
  40. Takashi Nakatsuka, Masahiro Nishihara, Kei-ichiro Mishiba, Hiroshi Hirano, Saburo Yamamura (2006)
    Two different transposable elements inserted in flavonoid 3′,5′-hydroxylase gene contribute to pink flower coloration in Gentiana scabra.
    Molecular Genetics and Genomics 275(3): 231-241
  41. Kei-ichiro Mishiba, Kei-ichi Tawada, Masahiro Mii (2006)
    Ploidy distribution in the explant tissue and the calluses induced during the initial stage of internode segment culture of Asparagus officinalis L.
    In Vitro Cellular and Developmental Biology – Plant 42(1): 83-88
  42. Kei-ichiro Mishiba, Masahiro Nishihara, Yoshiko Abe, Takashi Nakatsuka, Hiromi Kawamura, Katsuo Kodama, Toshikazu Takesawa, Jun Abe, Saburo Yamamura (2006)
    Production of dwarf potted gentian using wild-type Agrobacterium rhizogenes.
    Plant Biotechnology 23(1): 33-38
  43. Masahiro Nishihara, Takashi Nakatsuka, Keizo Hosokawa, Takahide Yokoi, Yoshiko Abe, Kei-ichiro Mishiba, Saburo Yamamura (2006)
    Dominant inheritance of white-flowered and herbicide-resistant traits in transgenic gentian plants.
    Plant Biotechnology 23(1): 25-31
  44. Takashi Nakatsuka, Masahiro Nishihara, Kei-ichiro Mishiba, Saburo Yamamura (2006)
    Heterologous expression of two gentian cytochrome P450 genes can modulate the intensity of flower pigmentation in transgenic tobacco plants.
    Molecular Breeding 17(2): 91-99
  45. Kei-ichiro Mishiba, Masahiro Nishihara, Takashi Nakatsuka, Yoshiko Abe, Hiroshi Hirano, Takahide Yokoi, Akiko Kikuchi, Saburo Yamamura (2005)
    Consistent transcriptional silencing of 35S-driven transgenes in gentian.
    Plant Journal 44(4): 541-556
  46. Takashi Nakatsuka, Masahiro Nishihara, Kei-ichiro Mishiba, Saburo Yamamura (2005)
    Two different mutations are involved in the formation of white-flowered gentian plants.
    Plant Science 169(5): 949-958
  47. Kei-ichiro Mishiba, Dong Poh Chin, Masahiro Mii (2005)
    Agrobacterium-mediated transformation of Phalaenopsis by targeting protocorms at an early stage after germination.
    Plant Cell Reports 24: 297-303
  48. Takashi Nakatsuka, Masahiro Nishihara, Kei-ichiro Mishiba, Saburo Yamamura (2005)
    Temporal expression of flavonoid biosynthesis-related genes regulates flower pigmentation in gentian plants.
    Plant Science 168(5): 1309-1318
  49. Keiko Ishikawa, Hitoshi Kuboki, Kei-ichiro Mishiba, Osamu Nomura (2001)
    Tetraploid bell pepper shows high in vitro pollen germination at 15˚C.
    HortScience 36(7): 1336
  50. Kei-ichiro Mishiba, Tomonori Okamoto, Masahiro Mii (2001)
    Increasing ploidy level in cell suspension cultures of Doritaenopsis by exogenous application of 2,4-dichlorophenoxyacetic acid.
    Physiologia Plantarum 112(1): 142-148
  51. Tadanori Nozaki, Kei-ichiro Mishiba, Masahiro Mii, Takato Koba (2000)
    Construction of synteny groups of Brassica alboglabra by RAPD markers and detection of chromosome aberrations and distorted transmission under the genetic background of B. campestris.
    Theoretical and Applied Genetics 101(4): 538-546
  52. Kei-ichiro Mishiba, Masahiro Mii (2000)
    Polysomaty analysis in diploid and tetraploid Portulaca grandiflora.
    Plant Science 156(2): 213-219
  53. Kei-ichiro Mishiba, Toshio Ando, Masahiro Mii, Hitoshi Watanabe, Hisashi Kokubun, Goro Hashimoto, Eduardo Marchesi (2000)
    Nuclear DNA content as an index character discriminating taxa in the genus Petunia sensu Jussieu (Solanaceae).
    Annals of Botany 85(5): 665-673
  54. Kei-ichiro Mishiba, Keiko Ishikawa, Osamu Tsujii, Masahiro Mii (2000)
    Efficient transformation of lavender (Lavandula latifolia Medicus) mediated by Agrobacterium.
    Journal of Horticultural Science & Biotechnology 75(3): 287-292
  55. Keiko Ishikawa, Kei-ichiro Mishiba, Hayato Yoshida, Osamu Nunomura (1997)
    Establishment of tetraploid plants of Capsicum annuum L. by colchicine treatment with the analysis of flow cytometry.
    Capsicum and Eggplant Newsletter 16: 44-47
  56. Keiko Ishikawa, Kei-ichiro Mishiba, Tsuyoshi Fujita, Kyoko Sato, Masahiro Mii (1996)
    Growth and betalain contents in two different strains of beet (Beta vulgaris L.) hairy root cultures.
    Japanese Journal of Food Chemistry 3(1): 43-46 (in Japanese)

 

【総説・著書】

  1. Masahiro Nishihara, Kei-ichiro Mishiba, Tomohiro Imamura, Hideyuki Takahashi, Takashi Nakatsuka (2015)
    Molecular breeding of Japanese gentians—Applications of genetic transformation, metabolome analyses, and genetic markers.
    In: Jan J. Rybczyński, Michael R. Davey, Anna Mikuła Eds., The Gentianaceae-Volume 2: Biotechnology and Applications, Springer, pp239-265
  2. Kei-ichiro Mishiba, Dong Poh Chin, Masahiro Mii (2007)
    Genetic Engineering of Orchids.
    In: Ashwani Kumar, Sudhir K. Sopory Eds., Recent Advances in Plant Biotechnology and Its Applications, I.K. International, pp288-302
  3. Kei-ichiro Mishiba, Masahiro Mii (2006)
    Polysomaty and Somaclonal Variation in Plants.
    In: Jaime A. Teixeira da Silva Eds., Floriculture, Ornamental and Plant Biotechnology Volume II, Global Science Books, pp262-270
  4. 三柴啓一郎、三位正洋 (1999) フローサイトメトリー自由自在 「植物研究への応用」(共著)、中内啓光監修、秀潤社、pp67-73

 

【研究報告書等】

  1. Kei-ichiro Mishiba, Yukihiro Nagashima, Noriko Hayashi, Nozomu Koizumi (2014) DNA fragmentation analysis. Bio-protocol 4(15): e1203
    http://www.bio-protocol.org/e1203
  2. Dong Poh Chin, Masahiro Mii, Kei-ichiro Mishiba (2007) Production of transgenic Phalaenopsis plants by introducing glutathione S-transferase gene into protocorms at an early stage after germination. Acta Horticulturae 743: 101-105
  3. 佐々木義則、三柴啓一郎、三位正洋 (2001) 二倍体と四倍体の交配によって得られたスギ、ヒノキF1個体のフローサイトメトリー分析による倍数性及び異数性の推定.日本林学会九州支部研究論文集 No.54: 69-70
  4. 佐々木義則、三柴啓一郎、三位正洋 (2000) スギ、ヒノキ異数体のフローサイトメトリー分析.日本林学会九州支部研究論文集 No.53: 59-60
  5. 佐々木義則、石塚涼子、三柴啓一郎、三位正洋 (1998) スギ、ヒノキ倍数体および自然突然変異体のフローサイトメトリー分析.大分県林業試験場研究時報 第24号: 1-16
  6. 佐々木義則、三柴啓一郎、三位正洋 (1997) フローサイトメトリー分析によるスギ、ヒノキの倍数性の判別.日本林学会九州支部研究論文集No.50: 55-56
  7. 佐々木義則、三柴啓一郎、三位正洋 (1997) フローサイトメトリーによるスギ、ヒノキの倍数性および核DNA量の測定.大分県林業試験場研究時報第23号: 1-8
  8. 佐々木義則、三柴啓一郎、三位正洋 (1997) フローサイトメトリーによるスギ、ヒノキの倍数性および核DNA量の測定.材木の育種特別号: 51-54

 

【研究費】

  1. 「植物のバイオマス分配に及ぼす物理環境の複合影響の解明とフェノミクス研究への応用」、科学研究費(基盤研究B)、2018~2020年度、分担(研究代表者:渋谷俊夫)
  2. 「アブラナ種子の油脂高蓄積を目的とした小胞体環境の改変技術開発」、松籟科学技術振興財団 第35回研究助成、2017年度、代表
  3. 「種子発達におけるIRE1活性化機構の解明と種子バイオマス改変技術の開発」、科学研究費(基盤研究C)、2017~2019年度、代表
  4. 「高等植物における新規サイレンシング機構の解明」、第29回 ノバルティス研究奨励金、2016年度、代表
  5. 「シロイヌナズナの種子発達におけるIRE1の役割の解明」、大阪府立大学大学院 生命環境科学研究科共同研究奨励金、2015年度、代表
  6. 「高等植物におけるRIDDの生理的意義の解明」、武田科学振興財団 ライフサイエンス研究奨励、2014年度、代表
  7. 「IRE1の欠損による種子大型化の原因解明」、科学研究費(基盤研究C)、2014~2016年度、代表
  8. 「種特異的な外来遺伝子発現抑制現象の解明」、科学研究費(若手研究B)、2011~2013年度、代表
  9. 「バイオエタノール生産に適した遺伝子組換えサトウキビの開発」、日揮・実吉奨学会研究助成、2010~2011年、代表
  10. 「光合成関連遺伝子の導入を目的としたサトウキビへの効率的な遺伝子導入系の開発」、大阪府立大学大学院奨励特別研究費、2009年度、代表
  11. 「リンドウに導入した35Sプロモーターのメチル化に関与する因子の探索」、科学研究費(若手研究B)、2008~2009年度、代表
  12. 「キクに内在するトランスポゾンを利用した新規突然変異誘発技術の開発」、大阪府立大学大学院 生命環境科学研究科特別奨励研究助成、2006年度、代表
  13. 「リンドウで見出された新規な外来遺伝子発現抑制現象の解明」、 科学研究費(若手研究B)、2006~2007年度、代表
  14. 「特定の高等植物にみられる新規な外来遺伝子発現抑制現象の解明」、住友財団 基礎科学研究助成、2005年度、代表